chimeric anti pd 1 mabs  (Jackson Immuno)

Journal: medRxiv

Article Title: Performance of three SARS-CoV-2 immunoassays, three rapid lateral flow tests and a novel bead-based affinity surrogate test for the detection of SARS-CoV-2 antibodies in human serum

doi: 10.1101/2021.02.07.21251062

Figure Lengend Snippet: Results of the ELISA assays plotted against the titers from the neutralization assay (n=63). Values exceeding the maximum respectively the minimum in a test were plotted at this maximum or minimum value (Mikrogen IgA: >150.12 U/ml; Mikrogen IgG: >133.93 U/ml; Euroimmun IgA: >8.54; SERION IgA: <5.0 U/ml; SERION IgG: <3.0 U/ml; >250.0 U/ml) Mikrogen: Mikrogen recomWell SARS-CoV-2 IgA / IgG (Mikrogen, Neuried, Germany), based on the nucleocapsid Euroimmun: Euroimmun SARS-COV-2 IgA / IgG (Euroimmun, Lübeck, Germany), based on S1 antigen SERION: SERION ELISA agile SARS-CoV-2 IgA / IgG (Institut Virion/Serion, Würzburg, Germany), based on the nucleocapsid as well as S1 and S2 antigen PRNT50: 50 % plaque reduction neutralization test. Method described in the methods section. PRNT50 < 20 were considered negative.

Article Snippet: A mouse/human chimeric monoclonal anti-SARS-CoV2-Spike-S1 antibody (Sino Biological, Beijing, China) was used as a positive control for all measurements.

Techniques: Enzyme-linked Immunosorbent Assay, Neutralization, Plaque Reduction Neutralization Test

Journal: Molecular Therapy

Article Title: DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure

doi: 10.1016/j.ymthe.2020.02.007

Figure Lengend Snippet: Characteristics of Anti-CTLA-4 and Anti-PD-1 mAb-Encoding pDNA Constructs

Article Snippet: Additionally, Absolute Antibody murinized the chimeric IgG1 anti-PD-1 mAb sequences, i.e., mutations were introduced in the variable regions to improve similarity to mouse germline sequences [p(CMV- m aPD-1-HC 1 ), p(CMV- m aPD-1-LC)].

Techniques: Plasmid Preparation

Journal: Molecular Therapy

Article Title: DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure

doi: 10.1016/j.ymthe.2020.02.007

Figure Lengend Snippet: Engineering of a DNA-Based Anti-CTLA-4 mAb and DNA-Based Anti-PD-1 mAb

Article Snippet: Additionally, Absolute Antibody murinized the chimeric IgG1 anti-PD-1 mAb sequences, i.e., mutations were introduced in the variable regions to improve similarity to mouse germline sequences [p(CMV- m aPD-1-HC 1 ), p(CMV- m aPD-1-LC)].

Techniques: